Flow cytometric nuclear DNA ploidy analysis was used to study pathologic stage C prostatic
adenocarcinoma (pT3, N0, M0) in 146 patients who underwent radical retropubic prostatectomy
and bilateral pelvic lymphadenectomy between 1967 and 1981. Of these tumors, 46% had
a DNA diploid pattern, 47% had a DNA tetraploid pattern, and 7% had a DNA aneuploid
pattern. Abnormal ploidy patterns were associated more frequently with histologic
high-grade tumors than with low-grade tumors. Considered alone, DNA ploidy pattern
showed a strong association with subsequent prognosis. The median interval to progression
for tumors with DNA tetraploid and DNA aneuploid patterns was 7.8 and 3.5 years, respectively.
For the DNA diploid tumors, only 23% progressed within 18 years, the longest follow-up.
At 10 years, only 10% of patients with DNA diploid tumors had died of prostatic cancer,
in comparison with 28% of the DNA tetraploid and 36% of the DNA aneuploid groups (P<0.01).
By analysis of a combination of histologic tumor grade and nuclear DNA ploidy pattern,
an even stronger association with prognosis was demonstrated. For the 38 patients
with histologic low-grade and DNA diploid tumors, progression-free survival was 92%
at 10 years, in comparison with 57% for 23 patients with low-grade DNA nondiploid
tumors. Patients with high-grade tumors had a poorer prognosis whether the DNA ploidy
pattern was diploid or nondiploid. Nuclear DNA ploidy pattern is an important and
independent prognostic variable for patients with pathologic stage C prostatic cancer
treated by radical prostatectomy.
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Mayo Clinic ProceedingsAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
REFERENCES
- The clinical spectrum of prostate cancer.J Urol. 1977; 117: 323-327
- Carcinoma of the prostate: a review.J Urol. 1978; 119: 1-8
- Prostatic carcinoma.Br Med J. 1985; 290: 875-876
- Prostatic carcinoma cell DNA content measured by flow cytometry and its relation to clinical outcome.Br J Surg. 1986; 73: 400-403
- The application of flow cytometry to the assessment of tumor cell heterogeneity and the grading of human prostatic cancer: preliminary results.J Urol. 1986; 135: 1194-1198
- Flow cytometry in assessing the clinical aggressiveness of genito-urinary neoplasms.World J Urol. 1987; 5: 108-122
- Flow cytometry of prostate cancer: relationship of DNA content to survival.Cancer Res. 1987; 47: 2504-2507
- Stage D1 prostatic adenocarcinoma: significance of nuclear DNA ploidy patterns studied by flow cytometry.Mayo Clin Proc. 1988; 63: 103-112
- Pathologic differentiation and prognosis of prostatic carcinoma.JAMA. 1969; 209: 1701-1703
- Prediction of prognosis for prostatic adenocarcinoma by combined histological grading and clinical staging.J Urol. 1974; 111: 58-64
- Radical retropubic prostatectomy and pelvic lymphadenectomy for high-stage cancer of the prostate.Cancer. 1981; 47: 1901-1910
- Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry.J Histochem Cytochem. 1983; 31: 1333-1335
- A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis.Cytometry. 1983; 3: 323-327
- Mathematical analysis of DNA distributions derived from flow microfluorometry.J Cell Biol. 1974; 60: 523-527
- Nonparametric estimation from incomplete observations.J Am Stat Assoc. 1958; 53: 457-481
- Asymptomatically efficient rank invariant test procedures.J R Stat Soc [A]. 1972; 135: 185-206
- Flow cytometric determination of ploidy in prostatic adenocarcinoma: a comparison with seminal vesicle involvement and histopathological grading as a predictor of clinical recurrence.J Urol. 1988; 140: 769-774
Article Info
Identification
Copyright
© 1989 Mayo Foundation for Medical Education and Research. Published by Elsevier Inc. All rights reserved.
